GATA2 Antibody
Rabbit Polyclonal
Catalog number: 11103-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, K-562 cells, mouse heart tissue, mouse liver tissue, Raji cells |
| Positive IHC detected in | human lymphoma |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 11103-1-AP(GATA2 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human lymphoma using 11103-1-AP(GATA2 Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using GATA2 antibody 11103-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | GATA2 fusion protein ag1557 | Full name | GATA binding protein 2 |
|
Calculated molecular weight |
466aa,49kd |
Observed molecular
weight |
60-66kd |
|
GenBank accession number |
BC018988 | Gene ID (NCBI) | 2624 |
| Gene symbol | GATA2 | Synonyms | FLJ45948; MGC2306; NFE1B |
Background
GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo. |
