GATA5 Polyclonal antibody

GATA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55433-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Transcription factor GATA-5, Transcription factor GATA 5, GATA-binding factor 5, GATA binding factor 5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 55433-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55433-1-AP (GATA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 55433-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55433-1-AP (GATA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 55433-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55433-1-AP (GATA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 55433-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55433-1-AP (GATA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 55433-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 55433-1-AP (GATA5 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 55433-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 55433-1-AP (GATA5 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 55433-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA5 antibody (55433-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (5)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55433-1-AP targets GATA5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	GATA binding protein 5
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	41 kDa
GenBank Accession Number	NM_080473
Gene Symbol	GATA5
Gene ID (NCBI)	140628
RRID	AB_2881330
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BWX5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for GATA5 antibody 55433-1-AP	Download protocol
IHC protocol for GATA5 antibody 55433-1-AP	Download protocol
WB protocol for GATA5 antibody 55433-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	IP	Cancers (Basel) Overexpression of GATA5 Inhibits Prostate Cancer Progression by Regulating PLAGL2 via the FAK/PI3K/AKT Pathway. Authors - Qinghua Wang View Article
human,mouse	WB,IF,IP	Biochem Biophys Res Commun Endothelial GATA5 positively regulates angiogenesis via cathepsin S-mediated Angpt2/Flk1 and MMP2/9 signaling pathways. Authors - Anmin Ren View Article
human	WB	Nat Commun Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy Authors - Wenjing Bai View Article
mouse	WB	Int J Biol Sci Cardiovascular Mettl3 Deficiency Causes Congenital Cardiac Defects and Postnatal Lethality in Mice Authors - Qianqian Feng View Article
human,mouse	WB	JACC Basic Transl Sci Disruption of Notch1 and Gata5 in Mice Leads to Congenital Aortic Valve Disease Authors - Jun Yasuhara View Article