GFAP Antibody
Rabbit Polyclonal
Catalog number: 16825-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | IF, IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | mouse, rat |
| Positive WB detected in | C6 cells |
| Positive IHC detected in | human brain |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
C6 cells were subjected to SDS PAGE followed by western blot with 16825-1-AP(GFAP Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human brain using 16825-1-AP(GFAP Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain using 16825-1-AP(GFAP Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | GFAP fusion protein ag10423 | Full name | glial fibrillary acidic protein |
|
Calculated molecular weight |
432aa,50kd |
Observed molecular
weight |
50kd |
|
GenBank accession number |
BC013596 | Gene ID (NCBI) | 2670 |
| Gene symbol | GFAP | Synonyms | FLJ45472 |
Background
GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes. |
