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GFRA2 Polyclonal antibody

GFRA2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55038-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using GFRA2 Polyclonal antibody (55038-1-AP)

    WB analysis of human placenta using 55038-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 55038-1-AP (GFRA2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GFRA2 Polyclonal antibody (55038-1-AP)

    IHC staining of human lung cancer using 55038-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55038-1-AP (GFRA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GFRA2 Polyclonal antibody (55038-1-AP)

    IHC staining of human lung cancer using 55038-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55038-1-AP (GFRA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55038-1-AP targets GFRA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GDNF family receptor alpha 2
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberNM_001495
Gene Symbol GFRA2
Gene ID (NCBI) 2675
RRIDAB_10859940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-52 kDa. This antibody is specific to GFRA2.

Protocols

Product Specific Protocols
WB protocol for GFRA2 antibody 55038-1-APDownload protocol
IHC protocol for GFRA2 antibody 55038-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols