GIP Polyclonal antibody

GIP Polyclonal Antibody for IHC, ELISA

Cat No. 18034-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, ELISA

Synonyms

gastric inhibitory polypeptide, GIP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllIHC

IHC staining of human pancreas using 18034-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas using 18034-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat pancreas using 18034-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse pancreas using 18034-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse pancreas using 18034-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat pancreas using 18034-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat pancreas using 18034-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 18034-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 18034-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive IHC detected in

human pancreas tissue, rat pancreas tissue, mouse pancreas tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18034-1-AP targets GIP in IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12634 Product name: Recombinant human GIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-153 aa of BC069663 Sequence: EKKEGHFSALPSLPVGSHAKVSSPQPRGPRYAEGTFISDYSIAMDKIHQQDFVNWLLAQKGKKNDWKHNITQREARALELASQANRKEEEAVEPQSSPAKNPSDEDLLRDLLIQELLACLLDQTNLCRLRSR Predict reactive species
Full Name	gastric inhibitory polypeptide
Calculated Molecular Weight	153 aa, 17 kDa
GenBank Accession Number	BC069663
Gene Symbol	GIP
Gene ID (NCBI)	2695
RRID	AB_2878484
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09681
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIP is an incretin hormone and belongs to the glucagon superfamily. GIP is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption.GIP stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion.