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GIP Polyclonal antibody

GIP Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18034-1-AP

Synonyms

gastric inhibitory polypeptide, GIP

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human pancreas tissue using GIP Polyclonal antibody (18034-1-AP)

    IHC staining of human pancreas using 18034-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using GIP Polyclonal antibody (18034-1-AP)

    IHC staining of human pancreas using 18034-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using GIP Polyclonal antibody (18034-1-AP)

    IHC staining of rat pancreas using 18034-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using GIP Polyclonal antibody (18034-1-AP)

    IHC staining of mouse pancreas using 18034-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using GIP Polyclonal antibody (18034-1-AP)

    IHC staining of mouse pancreas using 18034-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using GIP Polyclonal antibody (18034-1-AP)

    IHC staining of rat pancreas using 18034-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using GIP Polyclonal antibody (18034-1-AP)

    IHC staining of rat pancreas using 18034-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GIP Polyclonal antibody (18034-1-AP)

    IHC staining of human small intestine using 18034-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GIP Polyclonal antibody (18034-1-AP)

    IHC staining of human small intestine using 18034-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive IHC detected inhuman pancreas tissue, rat pancreas tissue, mouse pancreas tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18034-1-AP is GIP Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIP fusion protein Ag12634
Full Name gastric inhibitory polypeptide
Calculated Molecular Weight 153 aa, 17 kDa
GenBank Accession NumberBC069663
Gene Symbol GIP
Gene ID (NCBI) 2695
RRIDAB_2878484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIP is an incretin hormone and belongs to the glucagon superfamily. GIP is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption.GIP stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion.

Protocols

Product Specific Protocols
IHC protocol for GIP antibody 18034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Shashi (Verified Customer) (01-30-2020)

worked beautifully

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human small intestine Tissue