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  • Western Blot (WB) analysis of various lysates using GLE1 Polyclonal antibody (26466-1-AP)

    WB analysis using 26466-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using GLE1 Polyclonal antibody (26466-1-AP)

    WB analysis of A549 using 26466-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GLE1 Polyclonal antibody (26466-1-AP)

    WB analysis of MCF-7 using 26466-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GLE1 Polyclonal antibody (26466-1-AP)

    WB analysis of PC-3 using 26466-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26466-1-AP (GLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GLE1 Polyclonal antibody (26466-1-AP)

    IF Staining of HepG2 using 26466-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26466-1-AP (GLE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GLE1 Polyclonal antibody

GLE1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26466-1-AP
Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GLE1, GLE1 like protein, GLE1L, hGLE1, LCCS, LCCS1, Nucleoporin GLE1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells, PC-3 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26466-1-AP targets GLE1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24208

Product name: Recombinant human GLE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC030012

Sequence: MPSEGRCWETLKALRSSDKGRLCYYRDWLLRREDVLEECMSLPKLSSYSGWVVEHVLPHMQENQPLSETSPSSTSASALDQPSFVPKSPDASSAFSPASPATPNGTKGKDESQHTESM

Predict reactive species
Full Name GLE1 RNA export mediator homolog (yeast)
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 79-80 kDa
GenBank Accession NumberBC030012
Gene Symbol GLE1
Gene ID (NCBI) 2733
RRIDAB_2880525
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53GS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for GLE1 antibody 26466-1-APDownload protocol
WB protocol for GLE1 antibody 26466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Rashmi (Verified Customer) (09-25-2024)

Excellent Product

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
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