GLI4 Polyclonal antibody

GLI4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16551-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Zinc finger protein GLI4, Protein HKR4, Krueppel-related zinc finger protein 4, HKR4, GLI family zinc finger 4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIF/ICC

WB analysis of Jurkat using 16551-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 16551-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A549 using 16551-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using GLI4 antibody (16551-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	Jurkat cells, PC-3 cells, SH-SY5Y cells
Positive IF/ICC detected in	A549 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

16551-1-AP targets GLI4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9497 Product name: Recombinant human GLI4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC014165 Sequence: MAALGDIQESPSVPSPVSLSSPGTPGTQHHEPQLHLHGHQHGSPGSSPKVLSQPSDLDLQDVEEVEIGRDTFWPDSEPKPEQAPRSPGSQAPDEGAGGALRSLLRSLPRRARCSAGFGPESSAERPAGQPPGAVPCAQPRGAWRVTLVQQAAAGPEGAPERAAELGVNFGRSRQGSARGAKPHRCEACGKSFKYNSLLLKHQRIHTGEKPYACHECGKRFRGWSGFIQHHRIHTGEKPYECGQCGRAFSHSSHFTQHLRIHNGEKPYKCGECGQAFSQSSNLVRHQRLHTGEKPYACSQCGKAFIWSSVLIEHQRIHTGEKPYECSDCGKAFRGRSHFFRHLRTHTGEKPFACGACGKAFGQSSQLIQHQRVHYRE Predict reactive species
Full Name	GLI family zinc finger 4
Calculated Molecular Weight	376 aa, 41 kDa
Observed Molecular Weight	40-45 kDa
GenBank Accession Number	BC014165
Gene Symbol	GLI4
Gene ID (NCBI)	2738
RRID	AB_2109716
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P10075
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.