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Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, HeLa cells, K-562 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IF | See 1 publications below |
Product Information
10850-1-AP targets GLTP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1293 Product name: Recombinant human GLTP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC009932 Sequence: MALLAEHLLKPLPADKQIETGPFLEAVSHLPPFFDCLGSPVFTPIKADISGNITKIKAVYDTNPAKFRTLQNILEVEKEMYGAEWPKVGATLALMWLKRGLRFIQVFLQSICDGERDENHPNLIRVNATKAYEMALKKYHGWIVQKIFQAALYAAPYKSDFLKALSKGQNVTEEECLEKIRLFLVNYTATIDVIYEMYTQMNAELNYKV Predict reactive species |
| Full Name | glycolipid transfer protein |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC009932 |
| Gene Symbol | GLTP |
| Gene ID (NCBI) | 51228 |
| RRID | AB_2294627 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZD2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GLTP antibody 10850-1-AP | Download protocol |
| IHC protocol for GLTP antibody 10850-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci miR-196a Upregulation Contributes to Gefitinib Resistance through Inhibiting GLTP Expression. | ||
Arch Toxicol Peroxisome proliferator-activated receptor α attenuates high-cholesterol diet-induced toxicity and pro-thrombotic effects in mice. | ||
Hypertens Res Effects of hypertension and antihypertensive treatments on sulfatide levels in serum and its metabolism. | ||
Glycoconj J Age-dependent PPARα activation induces hepatic sulfatide accumulation in transgenic mice carrying the hepatitis C virus core gene. | ||
Biochim Biophys Acta Mol Cell Biol Lipids Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells. | ||
Mol Neurobiol Role of Gltp in Maturation of Oligodendrocytes Under the Regulation of Nkx2.2 |
