GNAT1 Polyclonal antibody

GNAT1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 55167-1-AP

Synonyms

CSNBAD3, GBT1, GNAT1, GNATR, Transducin alpha 1 chain



Tested Applications

Positive WB detected inmouse retina tissue, mouse eye tissue, rat retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55167-1-AP targets GNAT1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberNM_000172
Gene Symbol GNAT1
Gene ID (NCBI) 2779
RRIDAB_10858788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAT1, also named as GNATR, belongs to the G-alpha family and G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3). This antibody is specific to GNAT1.

Protocols

Product Specific Protocols
WB protocol for GNAT1 antibody 55167-1-APDownload protocol
IHC protocol for GNAT1 antibody 55167-1-APDownload protocol
IF protocol for GNAT1 antibody 55167-1-APDownload protocol
FC protocol for GNAT1 antibody 55167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Front Cell Dev Biol

Leveraging Zebrafish to Study Retinal Degenerations.

Authors - Juan M Angueyra
mouseWB,IF

FEBS J

Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice

Authors - Xiao Li
mouseWB

iScience

Identification of early-onset photoreceptor degeneration in transgenic mouse models of Alzheimer's disease.

Authors - Jie Zhang
zebrafishWB

J Biol Chem

Pathogenic Mutations in Retinitis Pigmentosa 2 Predominantly Result in Loss of RP2 Protein Stability in Human and Zebrafish.

Authors - Fei Liu
mouseWB

Sci Rep

Loss of PRCD alters number and packaging density of rhodopsin in rod photoreceptor disc membranes.

Authors - Emily R Sechrest
mouseIHC

eNeuro

Frmpd1 facilitates trafficking of G-protein transducin and modulates synaptic function in rod photoreceptors of mammalian retina

Authors - Christie K Campla