Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17375-1-AP targets GPBP1L1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11180 Product name: Recombinant human GPBP1L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-474 aa of BC022218 Sequence: MAQHDFVPAWLNFSTPQSAKSPTATFEKHGEHLPRGEGRFGVSRRRHNSSDGFFNNGPLRTAGDSWHQPSLFRHDSVDSGVSKGAYAGITGNPSGWHSSSRGHDGMSQRSGGGTGNHRHWNGSFHSRKGCAFQEKPPMEIREEKKEDKVEKLQFEEEDFPSLNPEAGKQHQPCRPIGTPSGVWENPPSAKQPSKMLVIKKVSKEDPAAAFSAAFTSPGSHHANGNKLSSVVPSVYKNLVPKPVPPPSKPNAWKANRMEHKSGSLSSSRESAFTSPISVTKPVVLASGAALSSPKESPSSTTPPIEISSSRLTKLTRRTTDRKSEFLKTLKDDRNGDFSENRDCDKLEDLEDNSTPEPKENGEEGCHQNGLALPVVEEGEVLSHSLEAEHRLLKAMGWQEYPENDENCLPLTEDELKEFHMKTEQLRRNGFGKNGFLQSRSSSLFSPWRSTCKAEFEDSDTETSSSETSDDDAWK Predict reactive species |
| Full Name | GC-rich promoter binding protein 1-like 1 |
| Calculated Molecular Weight | 474 aa, 52 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC022218 |
| Gene Symbol | GPBP1L1 |
| Gene ID (NCBI) | 60313 |
| RRID | AB_2232523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HC44 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPBP1L1 antibody 17375-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Joseph (Verified Customer) (04-30-2025) | Protein amount used for detection: 15–40 μg total protein per well Specificity: Validated with GPBP1L1 KO cells; some non-specific bands observed Molecular weight: Target band just below 70 kDa Blocking: 5% milk in PBS + 0.1% Tween 20 Primary antibody: 1:1500, 1 h at RT Wash: 3-4 × 5 min with PBS + 0.1% Tween 20
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