Tested Applications
| Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
11929-1-AP targets GPR137 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2611 Product name: Recombinant human GPR137 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 121-396 aa of BC033920 Sequence: VFKAKVKRRPEMSRGLLAVRGAFVGASLLFLLVNVLCAVLSHRRRAQPWALLLVRVLVSDSLFVICALSLAACLCLVARRAPSTSIYLEAKGTSVCQAAAMGGAMVLLYASRACYNLTALALAPQSRLDTFDYDWYNVSDQADLVNDLGNKGYLVFGLILFVWELLPTTLLVGFFRVHRPPQDLSTSHILNGQVFASRSYFFDRAGHCEDEGCSWEHSRGESTSMSGSLGSGSWYGAIGREPGWYGGSQTKTTPLLFSQVPGPGGHHHSLYSTPQT Predict reactive species |
| Full Name | G protein-coupled receptor 137 |
| Calculated Molecular Weight | 396 aa, 44 kDa |
| GenBank Accession Number | BC033920 |
| Gene Symbol | GPR137 |
| Gene ID (NCBI) | 56834 |
| RRID | AB_10694423 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96N19 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
G protein-coupled receptors (GPRs) are highly related to oncogenesis and cancer metastasis. G protein-coupled receptor 137 (GPR137) was initially reported as a novel orphan GPR about 10 years ago. GPR137 is ubiquitously expressed in the central nervous system (CNS), mainly in the hippocampus (PMID: 22025619). GPR137 is involved in the progression of human glioma, suggesting GPR137 as a potential oncogene of glioma cells (PMID: 24870220). Recent study shows that GPR137 plays an important role in colon cancer cell proliferation (PMID: 25301753).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPR137 antibody 11929-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biotechnol Appl Biochem G-protein-coupled receptor 137 accelerates proliferation of urinary bladder cancer cells in vitro.
| ||
Biotechnol Appl Biochem Knockdown of GPR137 by RNAi inhibits pancreatic cancer cell growth and induces apoptosis. | ||
Cell Biol Int Down-Regulation of G Protein-Coupled Receptor 137 by RNA Interference Inhibits Cell Growth of Two Hepatoma Cell Lines. | ||
Neurol Sci Inhibition of GPR137 expression reduces the proliferation and colony formation of malignant glioma cells. | ||
J Bone Oncol Small interfering RNA-mediated silencing of G-protein-coupled receptor 137 inhibits growth of osteosarcoma cells. | ||
Mol Med Rep RNA interference-mediated silencing of G protein-coupled receptor 137 inhibits human gastric cancer cell growth.
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