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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, human brain tissue, mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
20020-1-AP targets GPRASP1-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | G protein-coupled receptor associated sorting protein 1 |
| Calculated Molecular Weight | 157 kDa |
| Observed Molecular Weight | 157 kDa |
| GenBank Accession Number | NM_014710 |
| Gene Symbol | GPRASP1 |
| Gene ID (NCBI) | 9737 |
| RRID | AB_10694144 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5JY77 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPRASP1, also named as GASP, GASP1 and KIAA0443, belongs to the GPRASP family. GPRASP1 may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It is targets receptors for degradation in lysosomes. The antibody is specific to GPRASP1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPRASP1-Specific antibody 20020-1-AP | Download protocol |
| WB protocol for GPRASP1-Specific antibody 20020-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
