CSN1 Polyclonal antibody

CSN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11709-1-AP

Synonyms

GPS1, SGN1, JAB1-containing signalosome subunit 1, GPS-1, COPS1



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HT-1080 cells, Raji cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11709-1-AP targets CSN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSN1 fusion protein Ag2273
Full Name G protein pathway suppressor 1
Calculated Molecular Weight 491 aa, 55-60 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC000155
Gene Symbol CSN1
Gene ID (NCBI) 2873
RRIDAB_2232685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CSN1 antibody 11709-1-APDownload protocol
IHC protocol for CSN1 antibody 11709-1-APDownload protocol
IF protocol for CSN1 antibody 11709-1-APDownload protocol
IP protocol for CSN1 antibody 11709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

CSN1 facilitates proliferation and migration of hepatocellular carcinoma cells by upregulating cyclin A2 expression.

Authors - Hangwei Fu