Tested Applications
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12885-1-AP targets GSDMB in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3895 Product name: Recombinant human GSDMB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-385 aa of BC025682 Sequence: MFSVFEEITRIVVKEMDAGGDMIAVRSLVDADRFRCFHLVGEKRTFFGCRHYTTGLTLMDILDTDGDKWLDELDSGLQGQKAEFQILDNVDSTGELIVRLPKEITISGSFQGFHHQKIKISENRISQQYLATLENRKLKRELPFSFRSINTRENLYLVTETLETVKEETLKSDRQYKFWSQISQGHLSYKHKGQREVTIPPNRVLSYRVKQLVFPNKETMSAGLDIHFRGKTKSFPEGKSLGSEDSRNMKEKLEDMESVLKDLTEEKRKDVLNSLAKCLGKEDIRQDLEQRVSEVLISRELHMEDSDKPLLSSLFNAAGVLVEARAKAILDFLDALLELSEEQQFVAEALEKGTLPLLKDQVKSVMEQNWDELASSPPDMDYDPE Predict reactive species |
| Full Name | gasdermin B |
| Calculated Molecular Weight | 411 aa, 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC025682 |
| Gene Symbol | GSDMB |
| Gene ID (NCBI) | 55876 |
| RRID | AB_2114762 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAX9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GSDMB antibody 12885-1-AP | Download protocol |
| WB protocol for GSDMB antibody 12885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSDMB antibody (Cat# 12885-1-AP) has 13 citations published in journals including Cell Death & Disease, International Journal of Biological Sciences, Journal of Allergy and Clinical Immunology, Frontiers in Immunology, Molecular Metabolism, Journal of Biological Chemistry, and others. Research fields span pyroptosis, cancer biology (melanoma, bladder cancer, renal cell carcinoma, cholangiocarcinoma, oral squamous cell carcinoma), idiopathic pulmonary fibrosis, asthma, epithelial-mesenchymal transition, and tumor immune microenvironment characterization.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Clinical-mediated discovery of pyroptosis in CD8+ T cell and NK cell reveals melanoma heterogeneity by single-cell and bulk sequence | ||
Int J Biol Sci USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway. | ||
J Allergy Clin Immunol A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis. | ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma
| ||
Biol Direct A pyroptosis-related molecular signature stratifies prognosis and highlights GSDMB-mediated malignancy in intrahepatic cholangiocarcinoma. |
