Filter: AllWBIPIF/ICC

WB analysis using 66387-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 66387-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 66387-1-Ig
human colon tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 66387-1-Ig
Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 66387-1-Ig
K-562 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 66387-1-Ig
HL-60 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of TF-1 using 66387-1-Ig
TF-1 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 66387-1-Ig
A431 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of EC109 using 66387-1-Ig
EC109 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 66387-1-Ig
COLO 320 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 66387-1-Ig
Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human small intestine using 66387-1-Ig
human small intestine tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of THP-1 using 66387-1-Ig
IP result of anti-GSDMD (IP:66387-1-Ig, 4ug; Detection:66387-1-Ig 1:2000) with THP-1 cells lysate 1560 ug.
IF Staining of HeLa using 66387-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (66387-1-Ig, Clone: 2E6C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

GSDMD Monoclonal antibody

GSDMD Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 66387-1-Ig

Clone No.2E6C7

Publications(52)

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

2E6C7, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (52)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, A431 cells, COLO 320 cells, EC109 cells, HL-60 cells, human colon tissue, human small intestine tissue, K-562 cells, Raji cells, TF-1 cells, PC-3 cells, U2OS cells, A549 cells, HepG2 cells, Jurkat cells
Positive IP detected in	THP-1 cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 47 publications below
IHC	See 4 publications below
IF	See 13 publications below

Product Information

66387-1-Ig targets GSDMD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag16827 Product name: Recombinant human GSDMD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 135-484 aa of BC008904 Sequence: HERHLRQPEHKVLQQLRSRGDNVYVVTEVLQTQKEVEVTRTHKREGSGRFSLPGATCLQGEGQGHLSQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRSTSEGAWPQLPSGLSMMRCLHNFLTDGVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH Predict reactive species
Full Name	gasdermin D
Calculated Molecular Weight	484 aa, 53 kDa
Observed Molecular Weight	47-53 kDa
GenBank Accession Number	BC008904
Gene Symbol	GSDMD
Gene ID (NCBI)	79792
RRID	AB_2881763
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P57764
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.

Protocols

Product Specific Protocols
IF protocol for GSDMD antibody 66387-1-Ig	Download protocol
IP protocol for GSDMD antibody 66387-1-Ig	Download protocol
WB protocol for GSDMD antibody 66387-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cancer Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases Authors - Qiang Wang View Article
human	WB	PLoS Pathog An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS). Authors - Lan Lin View Article
human	WB	iScience ASC/inflammasome-independent pyroptosis in ovarian cancer cells through translational augmentation of caspase-1 Authors - Ozlem Calbay View Article
mouse	WB	Biochem Pharmacol Deficiency of purinergic P2X4 receptor alleviates experimental autoimmune hepatitis in mice Authors - Zejin Liu View Article
human	WB	Emerg Microbes Infect Acquiring high expression of suilysin enable non-epidemic Streptococccus suis to cause Streptococcal toxic shock-like syndrome (STSLS) through NLRP3 inflammasome hyperactivation. Authors - Lei Xu View Article
mouse	WB	Mol Med MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription Authors - Nan Zhao View Article