GTF2I Antibody
Rabbit Polyclonal
Catalog number: 10499-1-AP
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Applications
| Tested applications | ELISA, WB, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:200-1:2000 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10499-1-AP(GTF2I Antibody) at dilution of 1:800
Immunofluorescent analysis of Hela cells, using GTF2I antibody 10499-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | GTF2I fusion protein ag0773 | Full name | general transcription factor II, i |
|
Calculated molecular weight |
112kd |
Observed molecular
weight |
135kd |
|
GenBank accession number |
BC004472 | Gene ID (NCBI) | 2969 |
| Gene symbol | GTF2I | Synonyms | BAP-135; BAP135; BTKAP1; DIWS; FLJ38776; FLJ56355; IB291; SPIN; TFII-I; WBS; WBSCR6 |
Background
The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337]. |
