Tested Applications
| Positive WB detected in | L02 cells, HEK-293 cells, human liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13150-1-AP targets GYG2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3765 Product name: Recombinant human GYG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 141-471 aa of BC023152 Sequence: FQPSLHTHKLLLQHAMEHGSFDGADQGLLNSFFRNWSTTDIHKHLPFIYNLSSNTMYTYSPAFKQFGSSAKVVHFLGSMKPWNYKYNPQSGSVLEQGSVSSSQHQAAFLHLWWTVYQNNVLPLYKSVQAGEARASPGHTLCRSDVGGPCADSASGVGEPCENSTPSAGVPCANSPLGSNQPAQGLPEPTQIVDETLSLPEGRRSEDMIACPETETPAVITCDPLSQPSPQPADFTETETILQPANKVESVSSEETFEPSQELPAEALRDPSLQDALEVDLAVSVSQISIEEKVKELSPEEERRKWEEGRIDYMGKDAFARIQEKLDRFLQ Predict reactive species |
| Full Name | glycogenin 2 |
| Calculated Molecular Weight | 501 aa, 55 kDa |
| Observed Molecular Weight | 45 kDa, 66-70 kDa |
| GenBank Accession Number | BC023152 |
| Gene Symbol | GYG2 |
| Gene ID (NCBI) | 8908 |
| RRID | AB_2116111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15488 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GYG2 belongs to the glycogenin family. Glycogenin is a self-glucosylating protein involved in the initiation phase of glycogen biosynthesis. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, including the predominant band around 66 kDa and a light band around 45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GYG2 antibody 13150-1-AP | Download protocol |
| WB protocol for GYG2 antibody 13150-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
