Tested Applications
| Positive WB detected in | HL-60 cells |
| Positive IHC detected in | human lung tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25225-1-AP targets Galectin 10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18569 Product name: Recombinant human CLC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC119711 Sequence: MSLLPVPYTEAASLSTGSTVTIKGRPLVCFLNEPYLQVDFHTEMKEESDIVFHFQVCFGRRVVMNSREYGAWKQQVESKNMPFQDGQEFELSISVLPDKYQVMVNGQSSYTFDHRIKPEAVKMVQVWRDISLTKFNVSYLKR Predict reactive species |
| Full Name | Charcot-Leyden crystal protein |
| Calculated Molecular Weight | 142 aa, 16 kDa |
| Observed Molecular Weight | 13-16 kDa |
| GenBank Accession Number | BC119711 |
| Gene Symbol | Galectin 10 |
| Gene ID (NCBI) | 1178 |
| RRID | AB_2879973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q05315 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Galectin-10 (Gal-10), also called Charcot-Leyden Crystal (CLC) protein, is a member of the galectin superfamily of galactoside-binding lectins and is most prominently expressed in human eosinophils, where it forms the classic hexagonal bipyramidal Charcot-Leyden crystals that are considered hallmarks of eosinophilic inflammatory reactions.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Galectin 10 antibody 25225-1-AP | Download protocol |
| IHC protocol for Galectin 10 antibody 25225-1-AP | Download protocol |
| WB protocol for Galectin 10 antibody 25225-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Galectin 10 Polyclonal antibody (Cat# 25225-1-AP) has 4 total citations. These appear in Cells, Syst Biol Reprod Med, Int J Surg Pathol, and Oncol Lett. The associated research fields span chloride channel proteostasis and aldosteronism, differentially expressed proteins in ovarian endometriotic cysts, Langerhans cell histiocytosis pathology, and head and neck squamous cell carcinoma predictive modeling.
| Species | Application | Title |
|---|---|---|
Cells CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy. | ||
Syst Biol Reprod Med Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts. | ||
Int J Surg Pathol Langerhans Cell Histiocytosis With Unusual Hexagonal Crystals in Addition to Usual Charcot-Leyden Crystals. Report of a Patient With Possible Process of Crystal Formation and Clinical Significance of a "Necrotic" Change | ||
Oncol Lett Construction of a new predictive model in head and neck squamous cell carcinoma based on the investigation of extracellular matrix-associated genes. |
