HADHA AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, IP|
|Cited applications||IHC, WB|
|Species specificity||human, mouse; other species not tested.|
|Cited species||human, rat|
|Positive WB detected in||mouse kidney tissue, HEK-293 cells, A375 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF7 cells, MDA-MB-453s cells, mouse heart tissue, mouse liver tissue, Raji cells|
|Positive IHC detected in||human colon cancer|
|Positive IF detected in||HepG2 cells|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- IP result of HADHA antiibody (10758-1-AP for IP and Detection) with 293 cell lysate.
- mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA Antibody) at dilution of 1:1000
- Immunohistochemistry of paraffin-embedded human colon cancer using 10758-1-AP(HADHA Antibody) at Dilution 1:100 (under 10x lens)
- Immunofluorescent analysis of HepG2 cells, using HADHA antibody 10758-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||HADHA fusion protein ag1211||Full name||hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit|
|BC009235||Gene ID (NCBI)||3030|
|Gene symbol||HADHA||Synonyms||ECHA; GBP; HADH; LCHAD; MGC1728; MTPA; TP-ALPHA|
HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).This antibody is specific to HADHA.