HADHA Antibody
Rabbit Polyclonal
Catalog number: 10758-1-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | IHC, WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | human, rat |
| Positive WB detected in | mouse kidney tissue, HEK-293 cells, A375 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF7 cells, MDA-MB-453s cells, mouse heart tissue, mouse liver tissue, Raji cells |
| Positive IHC detected in | human colon cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of HADHA antiibody (10758-1-AP for IP and Detection) with 293 cell lysate.
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using 10758-1-AP(HADHA Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using HADHA antibody 10758-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | HADHA fusion protein ag1211 | Full name | hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit |
|
Calculated molecular weight |
83kd |
Observed molecular
weight |
70-79 kDa |
|
GenBank accession number |
BC009235 | Gene ID (NCBI) | 3030 |
| Gene symbol | HADHA | Synonyms | ECHA; GBP; HADH; LCHAD; MGC1728; MTPA; TP-ALPHA |
Background
HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).This antibody is specific to HADHA. |
