HDAC3 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | rat |
| Positive WB detected in | A431 cells, HeLa cells, HL-60 cells |
| Positive IHC detected in | human ovary tumor |
| Recommended dilution | WB: 1:200-1:1000 IHC: 1:20-1:200 |
IP = Immunoprecipitation
A431 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP(HDAC3 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human ovary tumor using 10255-1-AP(HDAC3 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human ovary tumor using 10255-1-AP(HDAC3 Antibody) at Dilution 1:50 (under 40x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | HDAC3 fusion protein ag0396 | Full name | histone deacetylase 3 |
|
Calculated molecular weight |
49kd |
Observed molecular
weight |
49kd |
|
GenBank accession number |
BC000614 | Gene ID (NCBI) | 8841 |
| Gene symbol | HDAC3 | Synonyms | HD3; RPD3; RPD3-2 |
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC3. |
