• Featured Product
  • KD/KO Validated

HECTD3 Polyclonal antibody

HECTD3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11487-1-AP

Synonyms

HECT domain containing 3, HECTD3, RP11 69J16.1



Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-453s cells
Positive IP detected inMDA-MB-453s cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11487-1-AP targets HECTD3 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HECTD3 fusion protein Ag1998
Full Name HECT domain containing 3
Calculated Molecular Weight 861 aa, 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC019105
Gene Symbol HECTD3
Gene ID (NCBI) 79654
RRIDAB_2117517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HECTD3(HECT domain-containing protein 3) may be involved in the regulation of mitotic metaphase/anaphase transition. It can specifically ubiquitinate TRIOBP and target it for proteasomal degradation and it can also mediates the ubiquitination of STX8. HECTD3 is a new oncogenic E3 ligase in human prostate cancer.( Li Y, etc. 2008) HECTD3 may facilitate cell cycle progression via regulating ubiquitination and degradation of Tara(PMID:18194665). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for HECTD3 antibody 11487-1-APDownload protocol
IP protocol for HECTD3 antibody 11487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Nat Commun

E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3.

Authors - Peng Gao
humanWB

Front Oncol

uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation.

Authors - Zhijian Zheng
humanWB

Cell Death Discov

HECTD3 promotes gastric cancer progression by mediating the polyubiquitination of c-MYC.

Authors - Guanghui Zhang
  • KD Validated
human,mouseWB,IP

Br J Cancer

HECTD3 regulates the tumourigenesis of glioblastoma by polyubiquitinating PARP1 and activating EGFR signalling pathway

Authors - Guanghui Zhang
  • KD Validated
humanCoIP

Cell Death Dis

Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells

Authors - Jing Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (01-23-2020)

Good, single band

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12