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HELB Polyclonal antibody

HELB Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 24700-1-AP

Synonyms

DNA helicase B, hDHB, HELB, helicase (DNA) B

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HELB Polyclonal antibody (24700-1-AP)

    WB analysis of Jurkat using 24700-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HELB Polyclonal antibody (24700-1-AP)

    WB analysis of HeLa using 24700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HELB Polyclonal antibody (24700-1-AP)

    WB analysis of HEK-293 using 24700-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using HELB Polyclonal antibody (24700-1-AP)

    IP experiment of HEK-293 using 24700-1-AP

    IP result of anti-HELB (IP:24700-1-AP, 4ug; Detection:24700-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HELB Polyclonal antibody (24700-1-AP)

    IF Staining of HeLa using 24700-1-AP

    Immunofluorescent analysis of HeLa cells using 24700-1-AP (HELB antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24700-1-AP is HELB Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HELB fusion protein Ag20335
Full Name helicase (DNA) B
Calculated Molecular Weight 1087 aa, 123 kDa
Observed Molecular Weight 120-140 kDa
GenBank Accession NumberBC157019
Gene Symbol HELB
Gene ID (NCBI) 92797
RRIDAB_2879680
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HELB antibody 24700-1-APDownload protocol
IF protocol for HELB antibody 24700-1-APDownload protocol
IP protocol for HELB antibody 24700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols