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  • KD/KO Validated

HERP Polyclonal antibody

HERP Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10813-1-AP

Synonyms

HERP, HERPUD1, KIAA0025, Mif1, SUP



Tested Applications

Positive WB detected inBxPC-3 cells, Tunicamycin treated HepG2 cells, HeLa cells, LNCaP cells, U2OS cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10813-1-AP targets HERP in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HERP fusion protein Ag1084
Full Name homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC008320
Gene Symbol HERPUD1
Gene ID (NCBI) 9709
RRIDAB_2248317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).

Protocols

Product Specific Protocols
WB protocol for HERP antibody 10813-1-APDownload protocol
IHC protocol for HERP antibody 10813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene.

Authors - Chengdong Liu
WB

J Alzheimers Dis

The Herp protein pathway is not involved in the pro-amyloidogenic effect of hyperhomocysteinemia.

Authors - Zhuo Jia-Min JM
IF

Biol Reprod

The unfolded protein response contributes to preimplantation mouse embryo death in the DDK syndrome.

Authors - Hao Lanping L
IHC

PLoS One

C/EBP-beta regulates endoplasmic reticulum stress-triggered cell death in mouse and human models.

Authors - Meir Ofir O
WB

Exp Cell Res

ER-localized protein-Herpud1 is a new mediator of IL-4-induced macrophage polarization and migration.

Authors - Yiman Li
  • KD Validated
humanWB

Mol Cell Biochem

Phe27Cys polymorphism of the human delta opioid receptor predisposes cells to compromised calcium signaling.

Authors - Tuusa Jussi T JT