Tested Applications
| Positive WB detected in | HEK-293 cells, mouse pancreas tissue, Jurkat cells, rat kidney tissue, HeLa cells, U2OS cells, mouse kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human prostate cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | N/A |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 13 publications below |
| IF | See 5 publications below |
Product Information
15857-1-AP targets Histone H2B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7811 Product name: Recombinant human Histone H2B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-126 aa of BC005827 Sequence: MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK Predict reactive species |
| Full Name | histone cluster 2, H2be |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 14-17 kDa |
| GenBank Accession Number | BC005827 |
| Gene Symbol | Histone H2B |
| Gene ID (NCBI) | 8349 |
| RRID | AB_10664929 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16778 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Histone H2B is a core component of the nucleosome, the fundamental unit of chromatin. It forms a dimer with Histone H2A, and two H2A-H2B dimers combine with a (H3-H4)₂ tetramer to create the histone octamer. The primary function of this structure is to package DNA into a compact and manageable form. However, H2B is also subject to various post-translational modifications (PTMs), such as ubiquitination, which serve as epigenetic marks to regulate gene expression by altering chromatin accessibility.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for Histone H2B antibody 15857-1-AP | Download protocol |
| WB protocol for Histone H2B antibody 15857-1-AP | Download protocol |
| IHC protocol for Histone H2B antibody 15857-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Nanotechnol Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells. | ||
Nat Biomed Eng Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues | ||
Oncogene HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis. | ||
Elife Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia. | ||
Mol Cell Proteomics Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells. | ||
Cell Discov USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Gunjita (Verified Customer) (01-06-2020) | N/A
|
FH Aurelie (Verified Customer) (11-19-2018) | strong signal around 20-25kda in mouse cortex (25µg) at 1:1000, (1:2000 should be ok as well)
|
