Tested Applications
| Positive WB detected in | HepG2 cells, A431 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55383-1-AP targets HLA-A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | major histocompatibility complex, class I, A |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41-44 kDa |
| GenBank Accession Number | NM_002116 |
| Gene Symbol | HLA-A |
| Gene ID (NCBI) | 3105 |
| RRID | AB_10888639 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04439 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HLA-A antibody 55383-1-AP | Download protocol |
| IHC protocol for HLA-A antibody 55383-1-AP | Download protocol |
| IP protocol for HLA-A antibody 55383-1-AP | Download protocol |
| WB protocol for HLA-A antibody 55383-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HLA-A Polyclonal antibody (Cat# 55383-1-AP) has 4 citations published in Drug Resist Updat, Front Pharmacol, Mol Cell Biochem, and J Virol. The research fields span hepatocellular carcinoma tumor immunogenicity, rifampicin-resistant spinal tuberculosis proteomics, EBV-associated gastric cancer immune escape, and HIV-1 MHC class I downregulation.
| Species | Application | Title |
|---|---|---|
Drug Resist Updat RNF26 regulating tumor immunogenicity of hepatocellular carcinoma by degrading GRP78 and instigating ER stress. | ||
Front Pharmacol Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis | ||
Mol Cell Biochem TAP1 promotes immune escape by activating JNK/STAT1/PD-L1 signaling in EBV-associated gastric cancer | ||
J Virol Two Functional Variants of AP-1 Complexes Composed of either γ2 or γ1 Subunits Are Independently Required for Major Histocompatibility Complex Class I Downregulation by HIV-1 Nef. |
Reviews
What customers say
The single review for 55383-1-AP is from Mounika Vallakati (Lot 00083445), who rated it 5 stars. She used the antibody at a 1:1000 dilution for Western Blot, Immunohistochemistry, and Immunofluorescence, describing it as "easy to work with" and "an asset to the project." Overall sentiment is strongly positive, highlighting ease of use across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (09-03-2026) | easy to work with, asset to the project!
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