Filter: AllWBIHC

WB analysis using 16109-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 16109-1-AP (HLA-DPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 16109-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16109-1-AP (HLA-DPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 16109-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16109-1-AP (HLA-DPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 16109-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16109-1-AP (HLA-DPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HLA-DPA1 Polyclonal antibody

Name: HLA-DPA1 Polyclonal antibody
Brand: Proteintech
SKU: 16109-1-AP

HLA-DPA1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16109-1-AP

Publications(5)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

DP(W3), DP(W4), HLA DP1A, HLA DPA1, HLA SB alpha chain, HLADP, HLADPA1, HLA-DPA1, HLASB, MHC class II DP3 alpha, MHC class II DPA1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (5)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Raji cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 3 publications below

Product Information

16109-1-AP targets HLA-DPA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9031 Product name: Recombinant human HLA-DPA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-224 aa of BC009956 Sequence: IKADHVSTYAAFVQTHRPTGEFMFEFDEDEQFYVDLDKKETVWHLEEFGRAFSFEAQGGLANIAILNNNLNTLIQRSNHTQAANDPPEVTVFPKEPVELGQPNTLICHIDRFFPPVLNVTWLCNGEPVTEGVAESLFLPRTDYSFHKFHYLTFVPSAEDVYDCRVEHWGLDQPLLKHWEAQEPIQMPETTETV Predict reactive species
Full Name	major histocompatibility complex, class II, DP alpha 1
Calculated Molecular Weight	260 aa, 29 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC009956
Gene Symbol	HLA-DPA1
Gene ID (NCBI)	3113
RRID	AB_1851387
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20036
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for HLA-DPA1 antibody 16109-1-AP	Download protocol
IHC protocol for HLA-DPA1 antibody 16109-1-AP	Download protocol
WB protocol for HLA-DPA1 antibody 16109-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Life Sci Activation of GABA receptor attenuates intestinal inflammation by modulating enteric glial cells function through inhibiting NF-κB pathway Authors - Ziteng Deng View Article
human	WB	Aging (Albany NY) HLA-DPA1 overexpression inhibits cancer progression, reduces resistance to cisplatin, and correlates with increased immune infiltration in lung adenocarcinoma Authors - Ke Shi View Article
human	IF	iScience Single-cell transcriptomics reveals the role of antigen presentation in liver metastatic breast cancer Authors - Xiaoshuang Wang View Article
human	IF	Ocul Surf Spatial transcriptomics and single-cell RNA-sequencing revealed dendritic cell-mediated inflammation in keratoconus Authors - Shiqi Luo View Article
human	WB	Front Immunol Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics Authors - Wei Liu View Article

Products

Applications

cGMP Proteins

Support

Services

Promotions

About Us

Antibodies

Immunoassays

Cytokines and Growth Factors

Nanobody-based Reagents

Supporting Tools and Reagents

Research Area

Genomics

WB analysis using 16109-1-AP

WB analysis using 16109-1-AP

IHC staining of human tonsillitis using 16109-1-AP

IHC staining of human tonsillitis using 16109-1-AP

HLA-DPA1 Polyclonal antibody

HLA-DPA1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Activation of GABA receptor attenuates intestinal inflammation by modulating enteric glial cells function through inhibiting NF-κB pathway

HLA-DPA1 overexpression inhibits cancer progression, reduces resistance to cisplatin, and correlates with increased immune infiltration in lung adenocarcinoma

Single-cell transcriptomics reveals the role of antigen presentation in liver metastatic breast cancer

Spatial transcriptomics and single-cell RNA-sequencing revealed dendritic cell-mediated inflammation in keratoconus

Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics