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WB analysis using 21702-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21702-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 21702-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21702-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 21702-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 21702-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 21702-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 21702-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 21702-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 21702-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using HLA-DRB5 antibody (21702-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 21702-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using HLA-DRB5 antibody (21702-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HLA-DRB5 Polyclonal antibody

HLA-DRB5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 21702-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

DR beta 2, DR beta 5, DR beta-5, DR2-beta-2, Dw2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Daudi cells, HeLa cells, HepG2 cells, HL-60 cells, Raji cells, Ramos cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

21702-1-AP targets HLA-DRB5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16307 Product name: Recombinant human HLA-DRB5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-266 aa of BC009234 Sequence: MVCLKLPGGSYMAKLTVTLMVLSSPLALAGDTRPRFLQQDKYECHFFNGTERVRFLHRDIYNQEEDLRFDSDVGEYRAVTELGRPDAEYWNSQKDFLEDRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPARTQTLQHHNLLVCSVNGFYPGSIEVRWFRNSQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRAQSESAQSKMLSGVGGFVLGLLFLGAGLFIYFKNQKGHSGLHPTGLVS Predict reactive species
Full Name	major histocompatibility complex, class II, DR beta 5
Calculated Molecular Weight	266 aa, 30 kDa
Observed Molecular Weight	30 kDa
GenBank Accession Number	BC009234
Gene Symbol	HLA-DRB5
Gene ID (NCBI)	3127
RRID	AB_2878905
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q30154
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for HLA-DRB5 antibody 21702-1-AP	Download protocol
IHC protocol for HLA-DRB5 antibody 21702-1-AP	Download protocol
WB protocol for HLA-DRB5 antibody 21702-1-AP	Download protocol