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HLF Polyclonal antibody

HLF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 12870-1-AP

Synonyms

hepatic leukemia factor, HLF

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of mouse liver using 12870-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of mouse brain using 12870-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of HEK-293 using 12870-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of K-562 using 12870-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of HepG2 using 12870-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, HepG2 cells, K-562 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12870-1-AP targets HLF in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLF fusion protein Ag3533
Full Name hepatic leukemia factor
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC036093
Gene Symbol HLF
Gene ID (NCBI) 3131
RRIDAB_11182935
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HLF antibody 12870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols