HMG20A Antibody
Rabbit Polyclonal
Catalog number: 12085-2-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human pancreas cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP(HMG20A Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human pancreas cancer using 12085-2-AP(HMG20A Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | HMG20A fusion protein ag2718 | Full name | high-mobility group 20A |
|
Calculated molecular weight |
347aa,40kd |
Observed molecular
weight |
45-50kd |
|
GenBank accession number |
BC021959 | Gene ID (NCBI) | 10363 |
| Gene symbol | HMG20A | Synonyms | FLJ10739; HMGX1; HMGXB1 |
Background
The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation |
