Tested Applications
| Positive WB detected in | A431 cells, A549 cells, HepG2 cells, LNCaP cells, mouse liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17643-1-AP targets HMGCS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11825 Product name: Recombinant human HMGCS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 171-520 aa of BC083514 Sequence: TGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTYHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH Predict reactive species |
| Full Name | 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble) |
| Calculated Molecular Weight | 520 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC083514 |
| Gene Symbol | HMGCS1 |
| Gene ID (NCBI) | 3157 |
| RRID | AB_2248359 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01581 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HMGCS1(Hydroxymethylglutaryl-CoA synthase, cytoplasmic) is also named as HMGCS and belongs to the HMG-CoA synthase family. The gene encodes a 520 amino acid protein which catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA and participates in the mevalonate pathway but its end product is cholesterol(PMID:20346956). The human and hamster enzymes are distinguished by differential inhibition by magnesium ion(PMID:2870496).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| IHC protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| IP protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| WB protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-HMGCS1 antibody (Cat# 17643-1-AP) has 68 total citations published in journals including Cancer Cell, Nature Cancer, Nature Communications, Neuron, Molecular Cell, Advanced Science, Cell Death & Disease, Nucleic Acids Research, and Journal of Biological Chemistry. Associated research fields encompass cholesterol and lipid metabolism, the mevalonate pathway, hepatocellular carcinoma, cancer metabolic reprogramming, ferroptosis, non-alcoholic fatty liver disease, neurodegeneration, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
Cancer Cell Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis
| ||
Nat Cancer Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism
| ||
Nat Cancer CHD1 loss reprograms SREBP2-driven cholesterol synthesis to fuel androgen-responsive growth and castration resistance in SPOP-mutated prostate tumors | ||
Nat Commun HMGCS1 drives cholesterol-dependent membrane repair and shields tumor cells from lymphocyte attack. | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
Metabolism Proteomic profiling reveals novel insights into hypercortisolism adrenal adenomas. |
Reviews
What customers say
All four reviewers gave this antibody a perfect 5-star rating. Users consistently report a clean, strong band at the expected molecular weight with no non-specific binding. It was successfully used for Western Blot at 1:1000 dilution and IHC at 1:200 across multiple cell lines and tissues, including HEK293T, HCT116, MEF cells, and mouse liver. One user noted that the target band intensity decreased upon cholesterol treatment, confirming functional relevance. Overall, reviewers describe it as a reliable, high-specificity antibody that delivers clear and reproducible results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Amy (Verified Customer) (02-06-2026) | Good antibody, clear band at expected molecular weight
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K (Verified Customer) (12-20-2020) | Used for western blotting (1:1000) and IHC (1:200), worked well for me
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H (Verified Customer) (07-09-2020) | This antibody is truly good. No non-specific band detected. The target band is very strong when 15ug of cell lysate was loaded.
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Boyan (Verified Customer) (07-08-2019) | This antibody recognises a clean, and clear band at the expected molecular weight. It goes down when you treat the cells with cholesterol.
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