Tested Applications
| Positive WB detected in | rat liver tissue, SMMC-7721 cells, HuH-7 cells |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22426-1-AP targets HNF1A in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18188 Product name: Recombinant human HNF1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 467-631 aa of BC104910 Sequence: PLHPSYQQPLMPPVQSHVTQSPFMATMAQLQSPHALYSHKPEVAQYTHTGLLPQTMLITDTTNLSALASLTPTKQVFTSDTEASSESGLHTPASQATTLHVPSQDPAGIQHLQPAHRLSASPTVSSSSLVLYQSSDSSNGQSHLLPSNHSVIETFISTQMASSSQ Predict reactive species |
| Full Name | HNF1 homeobox A |
| Calculated Molecular Weight | 631 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC104910 |
| Gene Symbol | HNF1A |
| Gene ID (NCBI) | 6927 |
| RRID | AB_2879104 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P20823 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HNF-1 , a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF1 as a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. It is required for the expression of several liver specific genes and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for HNF1A antibody 22426-1-AP | Download protocol |
| WB protocol for HNF1A antibody 22426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
HNF1A antibody (Cat# 22426-1-AP) has 18 citations published in journals including Cell Research, Theranostics, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cell Death & Disease, Acta Pharmacologica Sinica, and FEBS Letters. Research fields span hepatology (steatosis, liver injury, NAFLD), oncology (colorectal cancer, glioblastoma), lipid metabolism (cholesterol, atherosclerosis), diabetes (encephalopathy, β-cell function), virology (HBV, SARS-CoV-2), and transcriptional regulation.
| Species | Application | Title |
|---|---|---|
Cell Res Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer. | ||
Theranostics Small Heterodimer Partner Regulates Circadian Cytochromes p450 and Drug-Induced Hepatotoxicity. | ||
J Exp Clin Cancer Res ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer. | ||
Adv Sci (Weinh) Epigenetic Regulation of DAPK1 and Netrin-1 Drives Diabetic Encephalopathy | ||
Cell Death Dis CBP/p300 HAT maintains the gene network critical for β cell identity and functional maturity. | ||
Acta Pharmacol Sin Aloe-emodin exerts cholesterol-lowering effects by inhibiting proprotein convertase subtilisin/kexin type 9 in hyperlipidemic rats. |
Reviews
What customers say
One reviewer rated this product 5 stars, reporting successful use in Western Blot at a 1:2000 dilution on mouse liver tissue. They noted that the antibody works well with 1X TBST buffer. Overall, the feedback is positive, confirming reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kamal (Verified Customer) (07-05-2023) | Works with 1X TBST for WB.
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