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Tested Applications
| Positive WB detected in | rat liver tissue, HEK-293 cells, mouse kidney tissue, human liver tissue, mouse heart tissue, mouse liver tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15457-1-AP targets HOOK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7724 Product name: Recombinant human HOOK3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 370-718 aa of BC056146 Sequence: TYKRQVVELQNRLSEESKKADKLDFEYKRLKEKVDSLQKEKDRLRTERDSLKETIEELRCVQAQEGQLTTQGLMPLGSQESSDSLAAEIVTPEIREKLIRLQHENKMLKLNQEGSDNEKIALLQSLLDDANLRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDSVLLKKKLEEHLEKLHEANNELQKKRAIIEDLEPRFNNSSLKIEELQEALRKKEEEMKQMEERYKKYLEKAKSVIRTLDPKQNQGAAPEIQALKNQLQERDRLFHSLEKEYEKTKSQREMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQATSSRRSYPGHVQPATAR Predict reactive species |
| Full Name | hook homolog 3 (Drosophila) |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC056146 |
| Gene Symbol | HOOK3 |
| Gene ID (NCBI) | 84376 |
| RRID | AB_2120828 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VS8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HOOK3 antibody 15457-1-AP | Download protocol |
| IHC protocol for HOOK3 antibody 15457-1-AP | Download protocol |
| IP protocol for HOOK3 antibody 15457-1-AP | Download protocol |
| WB protocol for HOOK3 antibody 15457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Nat Commun PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport. | ||
Dev Cell The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance. | ||
J Cell Biol Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C. | ||
J Cell Biol Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons. | ||
Mol Cell Proteomics Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation | ||
iScience Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.
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