Tested Applications
| Positive WB detected in | EC109 cells, A431 cells, HaCaT cells |
| Positive IP detected in | mouse appendix tissue |
| Positive IHC detected in | mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| ChIP | See 3 publications below |
Product Information
18501-1-AP targets HOXA9 in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13350 Product name: Recombinant human HOXA9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC010023 Sequence: MATTGALGNYYVDSFLLGADAADELSVGRYAPGTLGQPPRQAATLAEHPDFSPCSFQSKATVFGASWNPVHAAGANAVPAAVYHHHHHHPYVHPQAPVAAAAPDGRYMRSWLEPTPGALSFAGLPSSRPYGIKPEPLSARRGDCPTLDTHTLSLTDYACGSPPVDREKQPSEGAFSENNAENESGGDKPPIDPNNPAANWLHARSTRKKRCPYTKHQTLELEKEFLFNMYLTRDRRYEVARLLNLTERQVKIWFQNRRMKMKKINKDRAKDE Predict reactive species |
| Full Name | homeobox A9 |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC010023 |
| Gene Symbol | HOXA9 |
| Gene ID (NCBI) | 3205 |
| RRID | AB_2861187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31269 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HOXA9, also known as HOX1G, belongs to the Abd-B homeobox family and transiently interacts with PRMT5 in TNF-alpha stimulated endothelial cells. HOXA9 is methylated on Arg-140 by PRMT5and methylation is critical for E-selectin induction. HOXA9 as a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. HOXA9 is required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. HOXA9 is 30 kDa, but methylation of HOXA9 is about 35-40 kDa. (PMID: 10082572)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HOXA9 antibody 18501-1-AP | Download protocol |
| IP protocol for HOXA9 antibody 18501-1-AP | Download protocol |
| WB protocol for HOXA9 antibody 18501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Epigenetics Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line | ||
iScience TIFAB accelerates MLL-AF9-Induced acute myeloid leukemia through upregulation of HOXA9. | ||
Cell Signal Aberrant TRPM4 expression in MLL-rearranged acute myeloid leukemia and its blockade induces cell cycle arrest via AKT/GLI1/Cyclin D1 pathway.
| ||
Cell Rep YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways. | ||
Sci Rep Transcription Factor HOXA9 is Linked to the Calcification and Invasion of Papillary Thyroid Carcinoma. |
