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  • Western Blot (WB) analysis of HeLa cells using HOXC10 Polyclonal antibody (20632-1-AP)

    WB analysis of HeLa using 20632-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20632-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HOXC10 Polyclonal antibody (20632-1-AP)

    WB analysis of HeLa using 20632-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20632-1-AP (HOXC10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HOXC10 Polyclonal antibody (20632-1-AP)

    IF Staining of HeLa using 20632-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20632-1-AP (HOXC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

HOXC10 Polyclonal antibody

HOXC10 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20632-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

HOX3I, Homeobox protein Hox-C10, Homeobox protein Hox-3I

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20632-1-AP targets HOXC10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14620

Product name: Recombinant human HOXC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC001293

Sequence: MTCPRNVTPNSYAEPLAAPGGGERYSRSAGMYMQSGSDFNCGVMRGCGLAPSLSKRDEGSSPSLALNTYPSYLSQLDSWGDPKAAYRLEQPVGRPLSSCSYPPSVKEENVCCMYSAEKRAKSGPEAALYSHPLPESCLGEHEVPVPSYYRASPSYSALDKTPHCSGANDFEAPFEQRASLNPRAEHLESPQLGGKVSFPETPKSDSQTPSPNEIKTEQSLAGPKGSPSESEKERAKAADSSPDTSDNEAKEEIKAENTTG

Predict reactive species
Full Name homeobox C10
Calculated Molecular Weight 342 aa, 38 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC001293
Gene Symbol HOXC10
Gene ID (NCBI) 3226
RRIDAB_10697666
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYD6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486)

Protocols

Product Specific Protocols
IF protocol for HOXC10 antibody 20632-1-APDownload protocol
WB protocol for HOXC10 antibody 20632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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