Filter: AllWBIHCIF/ICC

WB analysis using 20777-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 20777-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 20777-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 20777-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 20777-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 20777-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 20777-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).
IF Staining of Caco-2 using 20777-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HPDL antibody (20777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

HPDL Polyclonal antibody

Name: HPDL Polyclonal antibody
Brand: Proteintech
SKU: 20777-1-AP
Rating: 4.00 (1 reviews)

HPDL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20777-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HPD-like protein, Glyoxalase domain-containing protein 1, GLOXD1, EC:1.13.-.-, 4-hydroxyphenylpyruvate dioxygenase-like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, L02 cells
Positive IHC detected in	human stomach tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Caco-2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

20777-1-AP targets HPDL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14773 Product name: Recombinant human HPDL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-371 aa of BC007293 Sequence: MAAPALRLCHIAFHVPAGQPLARNLQRLFGFQPLASREVDGWRQLALRSGDAVFLVNEGAGSGEPLYGLDPRHAVPSATNLCFDVADAGAATRELAALGCSVPVPPVRVRDAQGAATYAVVSSPAGILSLTLLERAGYRGPFLPGFRPVSSAPGPGWVSRVDHLTLACTPGSSPTLLRWFHDCLGFCHLPLSPGEDPELGLEMTAGFGLGGLRLTALQAQPGSIVPTLVLAESLPGATTRQDQVEQFLARHKGPGLQHVGLYTPNIVEATEGVATAGGQFLAPPGAYYQQPGKERQIRAAGHEPHLLARQGILLDGDKGKFLLQVFTKSLFTEDTFFLELIQRQGATGFGQGNIRALWQSVQEQSARSQEA Predict reactive species
Full Name	4-hydroxyphenylpyruvate dioxygenase-like
Calculated Molecular Weight	371 aa, 39 kDa
Observed Molecular Weight	35-39 kDa
GenBank Accession Number	BC007293
Gene Symbol	HPDL
Gene ID (NCBI)	84842
RRID	AB_10697807
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96IR7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for HPDL antibody 20777-1-AP	Download protocol
IHC protocol for HPDL antibody 20777-1-AP	Download protocol
WB protocol for HPDL antibody 20777-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Gene Two novel heterozygous HPDL variants in a Chinese family with a neurodevelopmental disorder with progressive spasticity and brain white matter abnormalities Authors - Yuanxuan Ma View Article