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HTRA1 Polyclonal antibody

Name: HTRA1 Polyclonal antibody
Brand: Proteintech
SKU: 55011-1-AP

HTRA1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 55011-1-AP

Synonyms

ARMD7, High-temperature requirement A serine peptidase 1, HtrA, HtrA serine peptidase 1, HTRA1, L56, ORF480, PRSS11, Serine protease 11, Serine protease HTRA1

Validation Data Gallery

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AllWBIPIHCIF

WB analysis of L02 using 55011-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HAP1 cells using 55011-1-AP
HAP1 (WT and HTRA1 KO) lysates prepared with RIPA buffer, 25μg protein loaded. 55011-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
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HAP1 (WT and HTRA1 KO) lysates prepared with RIPA buffer, 25μg protein loaded. 55011-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
WB analysis of mouse brain using 55011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55011-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55011-1-AP (HTRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
$HAP1 lysates prepared and IP of APP performed using 2.0 μg of 55011-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.$
IP experiment of HAP1 cells using 55011-1-AP
HAP1 lysates prepared and IP of APP performed using 2.0 μg of 55011-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
×
HAP1 lysates prepared and IP of APP performed using 2.0 μg of 55011-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of L02 using 55011-1-AP
IP result of anti-HTRA1 (IP:55011-1-AP, 4ug; Detection:55011-1-AP 1:500) with L02 cells lysate 1400ug.
IHC staining of human placenta using 55011-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human placenta using 55011-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human placenta using 55011-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55011-1-AP (HTRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 55011-1-AP
Immunofluorescent analysis of HepG2 cells using 55011-1-AP (HTRA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 55011-1-AP

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Tested Applications

Positive WB detected in	L02 cells, mouse brain tissue, HAP1 cells, HepG2 cells
Positive IP detected in	L02 cells, HAP1 cells
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 4 publications below
IF	See 3 publications below

Product Information

55011-1-AP targets HTRA1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, Zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Full Name	HtrA serine peptidase 1
Calculated Molecular Weight	51 kDa
Observed Molecular Weight	35 kDa, 50 kDa, 42 kDa
GenBank Accession Number	NM_002775
Gene Symbol	HTRA1
Gene ID (NCBI)	5654
RRID	AB_10859830
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

Protocols

Product Specific Protocols
WB protocol for HTRA1 antibody 55011-1-AP	Download protocol
IHC protocol for HTRA1 antibody 55011-1-AP	Download protocol
IF protocol for HTRA1 antibody 55011-1-AP	Download protocol
IP protocol for HTRA1 antibody 55011-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse		Biomaterials Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm Authors - Craig Jun Kit Wong View Article
rat	WB,IF	Front Immunol Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling. Authors - Hong Xiang View Article
human	IHC,IF	FASEB J The "Matrisome" reveals the characterization of skin keloid microenvironment. Authors - Shikun Zhang View Article
Zebrafish	IHC,WB	Am J Pathol High Temperature Requirement A 1 (HTRA1) Causes Photoreceptor Cell Death in Zebrafish Disease Models. Authors - Yoshihito Oura View Article
human	IHC	Am J Pathol Proteolytic Degradation and Inflammation Play Critical Roles in Polypoidal Choroidal Vasculopathy. Authors - Sandeep Kumar View Article
mouse	WB	Bioengineered Long non-coding RNA lincRNA-erythroid prosurvival (EPS) alleviates cerebral ischemia/reperfusion injury by maintaining high-temperature requirement protein A1 (Htra1) stability through recruiting heterogeneous nuclear ribonucleoprotein L (HNRNPL). Authors - Haifeng Guo View Article

HTRA1 Polyclonal antibody

HTRA1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Validation Data Gallery

WB analysis of L02 using 55011-1-AP

WB analysis of HAP1 cells using 55011-1-AP

WB analysis of mouse brain using 55011-1-AP

WB analysis of mouse brain using 55011-1-AP

WB analysis of HepG2 using 55011-1-AP

WB analysis of mouse brain using 55011-1-AP

IP experiment of HAP1 cells using 55011-1-AP

IP experiment of L02 using 55011-1-AP

IHC staining of human placenta using 55011-1-AP

IHC staining of human placenta using 55011-1-AP

IHC staining of human placenta using 55011-1-AP

IF Staining of HepG2 using 55011-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm

Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling.

The "Matrisome" reveals the characterization of skin keloid microenvironment.

High Temperature Requirement A 1 (HTRA1) Causes Photoreceptor Cell Death in Zebrafish Disease Models.

Proteolytic Degradation and Inflammation Play Critical Roles in Polypoidal Choroidal Vasculopathy.

Long non-coding RNA lincRNA-erythroid prosurvival (EPS) alleviates cerebral ischemia/reperfusion injury by maintaining high-temperature requirement protein A1 (Htra1) stability through recruiting heterogeneous nuclear ribonucleoprotein L (HNRNPL).