Human HSP70 ELISA Kit0 Publications

Catalog number: KE00059

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Freight/Packing £00.00

Size: 09µ/gram

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Product name:
Human HSP70 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants, Urine

Assay type:

1.0 pg/mL

31.25-2000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Urine 86% 76%-98%
Human plasma 104% 92%-124%
Cell culture supernatants 97% 80%-123%

Samplenmean ( pg/mL)SDCV%
1 20 182.3 7.7 4.2
2 20 448.4 18.2 4.1
3 20 1,627.2 102.4 6.3

Samplenmean ( pg/mL)SDCV%
1 24 181.9 11.1 6.1
2 24 465.4 23.4 5.0
3 24 1,639.4 90.5 5.5

Product overview:
KE000059 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSP70 ELISA kit is to be used to detect and quantify protein levels of endogenous HSP70. The assay recognizes human HSP70. A polyclonal antibody specific for HSP70 has been pre-coated onto the microwells. The HSP70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for HSP70 is added to detect the captured HSP70 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B

HSP70 is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, refolding, transport, and targeting. Expression of HSP70 protein can be highly activated by various stressful stimuli. Significant up-regulation of HSP70 has been found in various tumors. Increased serum HSP70 levels are also associated with progression of multiple diseases, including atherosclerosis, heart failure, and diabetes.

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