IDH1 Antibody
Rabbit Polyclonal
Catalog number: 12332-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | MCF7 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, DU 145 cells |
| Positive IHC detected in | human kidney |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-IDH1 (12332-1-AP for IP and Detection) with MCF7 cell.
MCF7 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human kidney using 12332-1-AP(IDH1 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney using 12332-1-AP(IDH1 Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | IDH1 fusion protein ag2986 | Full name | isocitrate dehydrogenase 1 (NADP+), soluble |
|
Calculated molecular weight |
414aa,47kd |
Observed molecular
weight |
47kd |
|
GenBank accession number |
BC012846 | Gene ID (NCBI) | 3417 |
| Gene symbol | IDH1 | Synonyms | IDCD; IDH; IDP; IDPC; PICD |
Background
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).This antibody is specific to IDH1. |
