Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18013-1-AP targets IFN-alpha in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12533 Product name: Recombinant human IFNA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-189 aa of BC074928 Sequence: CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE Predict reactive species |
| Full Name | IFNA1 |
| Calculated Molecular Weight | 189 aa, 22 kDa |
| GenBank Accession Number | BC074928 |
| Gene Symbol | IFN alpha1 |
| Gene ID (NCBI) | 3439 |
| RRID | AB_2878481 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01562 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFNA1 (IFN-alpha) is a member of the Type I IFN (1) family best known for their antiviral activity. It is a key cytokine regulating the activity of B cells, T-helper cells (Th cells), cDCs and natural killer cells (NK cells). IFN-alpha induces B cell maturation into plasma cells and immunoglobulin production. IFN-alpha plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). IFN-alpha was the first cytokine to show clinical benefit in the treatment of certain types of cancer, including melanoma, chronic myelogenous leukemia, and renal cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFN-alpha antibody 18013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IFN-alpha Polyclonal antibody (Cat# 18013-1-AP) has 22 citations across journals including Nature Communications, Advanced Science, Cell Death & Disease, Cancer Letters, Oncogene, mBio, Journal for ImmunoTherapy of Cancer, Phytomedicine, and others. Research fields span immunology, oncology, virology, autoimmune disease, and inflammatory disorders, with applications in WB, IHC, and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Nat Commun Airway immune profiles and therapeutic implications of IGF1 in eosinophilic granulomatosis with polyangiitis. | ||
Adv Sci (Weinh) Tubule-Derived IFN-α Promotes GSDMD-Mediated Macrophage Pyroptosis to Drive Renal Inflammation and Fibrosis Through JAK2/STAT2 Activation. | ||
Cell Death Dis Enhancing immunotherapy efficacy in colorectal cancer: targeting the FGR-AKT-SP1-DKK1 axis with DCC-2036 (Rebastinib) | ||
Cancer Lett PRTN3 promotes IL33/Treg-mediated tumor immunosuppression by enhancing the M2 polarization of tumor-associated macrophages in lung adenocarcinoma | ||
J Immunother Cancer Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma. | ||
Phytomedicine NOX2 inhibitor baicalein alleviates rheumatoid arthritis and inactivating mitochondrial oxidative stress-mediated cGAS-STING signaling in fibroblast-like synoviocytes. |
