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Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
26952-1-AP targets IFRD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25057 Product name: Recombinant human IFRD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 413-442 aa of BC007265 Sequence: RFEKHLYNAAAFKARTKARSRVRDKRADIL Predict reactive species |
| Full Name | interferon-related developmental regulator 2 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC007265 |
| Gene Symbol | IFRD2 |
| Gene ID (NCBI) | 7866 |
| RRID | AB_2880700 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12894 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFRD2, or interferon-related developmental regulator 2, is a protein that has been identified as a novel factor capable of translationally inactivating ribosomes. It plays a role in regulating gene expression by directly binding to 80S ribosomes, which precludes the binding of mRNA and P-site tRNA, thereby inhibiting translation
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IFRD2 antibody 26952-1-AP | Download protocol |
| WB protocol for IFRD2 antibody 26952-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
