IFT88 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, IP|
|Cited applications||IF, WB|
|Species specificity||human, mouse, rat; other species not tested.|
|Cited species||canine, human, mouse|
|Positive WB detected in||HEK-293 cells, A549 cells, HepG2 cells, Jurkat cells, K-562 cells, mouse kidney tissue, mouse testis tissue|
|Positive IHC detected in||human pancreas cancer|
|Recommended dilution||WB: 1:200-1:2000|
IP = Immunoprecipitation
- IP result of IFT88(13967-1-AP for IP; 60227-1-Ig for detection).
- IP and WB result of IFT88(13967-1-AP) from Dr. Corbit, Kevin. Knockout cells as control.
- IF result of IFT88(13967-1-AP) from Dr. Corbit, Kevin.
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 13967-1-AP(IFT88 Antibody) at dilution of 1:800
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 13967-1-AP(IFT88 Antibody) at Dilution 1:100 (under 10x lens)
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 13967-1-AP(IFT88 Antibody) at Dilution 1:100 (under 40x lens)
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||IFT88 fusion protein ag4980||Full name||intraflagellar transport 88 homolog (Chlamydomonas)|
|BC030776||Gene ID (NCBI)||8100|
|Gene symbol||IFT88||Synonyms||D13S1056E; DAF19; MGC26259; RP11-172H24.2; TG737; TTC10; hTg737|
IFT88 (intraflagellar transport protein 88), also known as TG737 or TTC10 (TPR repeat protein 10), is involved in primary cilium biogenesis. Cilia are organelles that play diverse roles, from ﬂuid movement to sensory reception. Mutations in IFT88/Tg737 of mouse cause a wide spectrum of phenotypes, including random left-right axis speciﬁcation, polycystic kidney disease, liver and pancreatic defects, hydrocephalus, and skeletal patterning abnormalities. IFT88 also play important roles in assembly and maintenance of photoreceptor outer segment. Additionally, IFT88 is thought to function as a centrosomal protein that may regulate the G1 to S phase cell cycle transition in non-ciliated cells.IFT88 is required for spindle orientation in mitosis, the depletion of IFT88 induces mitotic defects in human cultured cells. This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.