Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60256-1-Ig targets IGBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6782 Product name: Recombinant human IGBP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-339 aa of BC004137 Sequence: MAAEDELQLPRLPELFETGRQLLDEVEVATEPAGSRIVQEKVFKGLDLLEKAAEMLSQLDLFSRNEDLEEIASTDLKYLLVPAFQGALTMKQVNPSKRLDHLQRAREHFINYLTQCHCYHVAEFELPKTMNNSAENHTANSSMAYPSLVAMASQRQAKIQRYKQKKELEHRLSAMKSAVESGQADDERVREYYLLHLQRWIDISLEEIESIDQEIKILRERDSSREASTSNSSRQERPPVKPFILTRNMAQAKVFGAGYPSLPTMTVSDWYEQHRKYGALPDQGIAKAAPEEFRKAAQQQEEQEEKEEEDDEQTLHRAREWDDWKDTHPRGYGNRQNMG Predict reactive species |
| Full Name | immunoglobulin (CD79A) binding protein 1 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC004137 |
| Gene Symbol | IGBP1 |
| Gene ID (NCBI) | 3476 |
| RRID | AB_2881377 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P78318 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGBP1 antibody 60256-1-Ig | Download protocol |
| WB protocol for IGBP1 antibody 60256-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
