IGFBP3 Antibody
Rabbit Polyclonal
Catalog number: 10189-2-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells, A2780 cells |
| Recommended dilution | WB: 1:200-1:1000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 10189-2-AP(IGFBP3 Antibody) at dilution of 1:300
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | IGFBP3 fusion protein ag0242 | Full name | insulin-like growth factor binding protein 3 |
|
Calculated molecular weight |
32kd |
Observed molecular
weight |
37-45kd |
|
GenBank accession number |
BC000013 | Gene ID (NCBI) | 3486 |
| Gene symbol | IGFBP3 | Synonyms | BP-53; IBP3 |
Background
Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. |
