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Tested Applications
| Positive WB detected in | Jurkat cells, MOLT-4 cells, SKOV-3 cells, THP-1 cells |
| Positive IP detected in | mouse appendix tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 7 publications below |
| IF | See 3 publications below |
Product Information
19961-1-AP targets IGFBP7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | insulin-like growth factor binding protein 7 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | NM_001553 |
| Gene Symbol | IGFBP7 |
| Gene ID (NCBI) | 3490 |
| RRID | AB_2878630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16270 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGFBP7, also named as MAC25, PSF, IBP-7 and TAF, binds IGF-I and IGF-II with a relatively low affinity. IGFBP7 stimulates prostacyclin (PGI2) production. IGFBP7 stimulates cell adhesion.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGFBP7 antibody 19961-1-AP | Download protocol |
| IP protocol for IGFBP7 antibody 19961-1-AP | Download protocol |
| WB protocol for IGFBP7 antibody 19961-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Am Soc Nephrol Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI. | ||
Cancer Lett IGFBP7-AS1 is a p53-responsive long noncoding RNA downregulated by Epstein-Barr virus that contributes to viral tumorigenesis. | ||
J Cell Mol Med IGFBP7 regulates cell proliferation and migration through JAK/STAT pathway in gastric cancer and is regulated by DNA and RNA methylation | ||
Hum Genet MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract. | ||
Heliyon IGFBP7 promotes gastric cancer by facilitating epithelial-mesenchymal transition of gastric cells |
