IgG heavy chain Polyclonal antibody

human IgG heavy chain Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, RIP, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16402-1-AP

Synonyms

human IgG heavy chain, IgG Fc, IGHG, IGHG4, Immunoglobulin heavy chain gamma



Tested Applications

Positive WB detected inhuman plasma tissue, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16402-1-AP targets human IgG heavy chain in WB, RIP, IP, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen human IgG heavy chain fusion protein Ag9519
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Calculated Molecular Weight 473 aa, 52 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC025985
Gene Symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2878254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGHG4 has some fragments with MW 36 kDa (http://www.uniprot.org/uniprot/P01861) and 52 kDa (http://www.ncbi.nlm.nih.gov/protein/CAE45900). This antibody detects all the Heavy chain of human immunoglobulin gamma (IgG), IgG1, IgG2, IgG3, IgG4 included.

Protocols

Product Specific Protocols
WB protocol for human IgG heavy chain antibody 16402-1-APDownload protocol
IHC protocol for human IgG heavy chain antibody 16402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia.

Authors - Franc Llorens
humanIHC

Neuropathol Appl Neurobiol

Plasma total prion protein as a potential biomarker for neurodegenerative dementia: diagnostic accuracy in the spectrum of prion diseases.

Authors - Franc Llorens
humanCoIP

Mol Oncol

Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer.

Authors - Chao Fang
WB

Oncol Rep

Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells.

Authors - Li Liu

Int J Mol Sci

Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling.

Authors - Zhifeng Wu
RIP

Front Genet

A Circ-0007022/miR-338-3p/Neuropilin-1 Axis Reduces the Radiosensitivity of Esophageal Squamous Cell Carcinoma by Activating Epithelial-To-Mesenchymal Transition and PI3K/AKT Pathway.

Authors - Junpeng Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Pratheepa (Verified Customer) (06-11-2021)

The antibody works excellent. But the specificity is not restricted to human cells. It picks up mouse cell line also.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Adipose stem cell, Mouse microglial cell
human IgG heavy chain Antibody Immunofluorescence validation (1:200 dilution) in Adipose stem cell, Mouse microglial cell (Cat no:16402-1-AP)