Tested Applications
| Positive WB detected in | HL-60 cells, human brain tissue, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
23945-1-AP targets IGHMBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21057 Product name: Recombinant human IGHMBP2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 642-993 aa of BC105088 Sequence: YLDDIVPENYSHENSQGSSHAATKPQGPAASTRTGSQRQEGGQEAAAPARQGRKKPAGKSLASEAPSQPSLNGGSPEGVESQDGVDHFRAMIVEFMASKKMQLEFPPSLNSHDRLRVHQIAEEHGLRHDSSGEGKRRFITVSKRAPRPRAALGPPAGTGGPAPLQPVPPTPAQTEQPPREQRGPDQPDLRTLHLERLQRVRSAQGQPASKEQQASGQQKLPEKKKKKAKGHPATDLPTEEDFEALVSAAVKADNTCGFAKCTAGVTTLGQFCQLCSRRYCLSHHLPEIHGCGERARAHARQRISREGVLYAGSGTKNGSLDPAKRAQLQRRLDKKLSELSNQRTSRRKERGT Predict reactive species |
| Full Name | immunoglobulin mu binding protein 2 |
| Calculated Molecular Weight | 993 aa, 109 kDa |
| Observed Molecular Weight | 109 kDa |
| GenBank Accession Number | BC105088 |
| Gene Symbol | IGHMBP2 |
| Gene ID (NCBI) | 3508 |
| RRID | AB_2879371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P38935 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGHMBP2 also named as immunoglobulin mu binding protein 2 is a 993 amino-acid protein, which localizes in nuclear and cytoplasm. The molecular weight of IGHMBP2 is 109 kDa. IGHMBP2 belongs to the DNA2/NAM7 helicase family. IGHMBP2 also acts as a transcriptional regulator and is necessary for transcriptional activation of the flounder liver-type antifreeze protein gene. IGHMBP2 exists as a homooligomer and is part of the cytosolic ribonucleoprotein complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGHMBP2 antibody 23945-1-AP | Download protocol |
| WB protocol for IGHMBP2 antibody 23945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
bioRxiv IGHMBP2 deletion suppresses translation and activates the integrated stress response | ||
Life Sci Alliance IGHMBP2 deletion suppresses translation and activates the integrated stress response |
