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Tested Applications
| Positive WB detected in | human plasma tissue, mouse spleen tissue, human plasma |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
13688-1-AP targets IGJ in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4559 Product name: Recombinant human IGJ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-159 aa of BC038982 Sequence: MKNHLLFWGVLAVFIKAVHVKAQEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD Predict reactive species |
| Full Name | immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides |
| Calculated Molecular Weight | 158 aa, 18 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC038982 |
| Gene Symbol | IGJ |
| Gene ID (NCBI) | 3512 |
| RRID | AB_2877973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01591 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGJ antibody 13688-1-AP | Download protocol |
| IP protocol for IGJ antibody 13688-1-AP | Download protocol |
| WB protocol for IGJ antibody 13688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides. | ||
Int Immunopharmacol Integrative analysis-based identification and validation of a prognostic immune cell infiltration-based model for patients with advanced gastric cancer. | ||
J Biotechnol Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells. | ||
Plant Mol Biol Plant-derived secretory component gives protease-resistance to Shiga toxin 1-specific dimeric IgA. | ||
Oncol Lett Oridonin induces apoptosis in HGC-27 cells by activating the JNK signaling pathway. |
