Tested Applications
| Positive WB detected in | human brain tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
14003-1-AP targets IGSF11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IGSF11 fusion protein Ag5094 Predict reactive species |
| Full Name | immunoglobulin superfamily, member 11 |
| Calculated Molecular Weight | 430 aa, 46 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC034411 |
| Gene Symbol | IGSF11 |
| Gene ID (NCBI) | 152404 |
| RRID | AB_10603368 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5DX21 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGSF11 antibody 14003-1-AP | Download protocol |
| IHC protocol for IGSF11 antibody 14003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurosci Lett Promotion of differentiation in developing mouse cerebellar granule cells by a cell adhesion molecule BT-IgSF.
| ||
Medicine (Baltimore) Prediction of lung squamous cell carcinoma immune microenvironment and immunotherapy efficiency with pyroptosis-derived genes |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Dibyendu (Verified Customer) (04-30-2025) | I am writing regarding catalog number [A14003-1-AP] against [IGSF11]. Unfortunately, this antibody has proven unsuitable for our Western blot applications. In our hands it produces multiple non-specific bands across the entire cell lysate, and we are unable to distinguish any difference between knockdown and wild-type samples. As a result, we cannot reliably identify the target protein band, and the reagent has not yielded any usable data. Due to the promiscuity of the antibody we are even unsure of the results obtained from our IHC results using human cancer tissue samples. Could you please advise on a suitable alternative antibody clone or arrange for a refund? We appreciate your assistance in resolving this issue swiftly. Thank you for your prompt attention.
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