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HELIOS Polyclonal antibody

HELIOS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13554-1-AP

Synonyms

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HELIOS Polyclonal antibody (13554-1-AP)

    WB analysis of Jurkat using 13554-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13554-1-AP (HELIOS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using HELIOS Polyclonal antibody (13554-1-AP)

    WB analysis of human placenta using 13554-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13554-1-AP (HELIOS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using HELIOS Polyclonal antibody (13554-1-AP)

    IP experiment of Jurkat using 13554-1-AP

    IP result of anti-HELIOS (IP:13554-1-AP, 4ug; Detection:13554-1-AP 1:300) with Jurkat cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HELIOS Polyclonal antibody (13554-1-AP)

    IHC staining of human lymphoma using 13554-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13554-1-AP (HELIOS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HELIOS Polyclonal antibody (13554-1-AP)

    IHC staining of human lymphoma using 13554-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13554-1-AP (IKZF2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HELIOS Polyclonal antibody (13554-1-AP)

    IF Staining of HeLa using 13554-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13554-1-AP (HELIOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, human placenta tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13554-1-AP targets HELIOS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HELIOS fusion protein Ag4357
Full Name IKAROS family zinc finger 2 (Helios)
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC028936
Gene Symbol IKZF2
Gene ID (NCBI) 22807
RRIDAB_2124959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ikaros DNA-binding proteins, characterized by two highly conserved zinc finger domains that mediate DNA (N-terminal domain) and protein binding (C-terminal domain), consists of 5 members and one of them is IKZF2 [PMID:1439790]. IKZF2's expression is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685).

Protocols

Product Specific Protocols
WB protocol for HELIOS antibody 13554-1-APDownload protocol
IHC protocol for HELIOS antibody 13554-1-APDownload protocol
IF protocol for HELIOS antibody 13554-1-APDownload protocol
IP protocol for HELIOS antibody 13554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Immunohorizons

Clonal Bifurcation of Foxp3 Expression Visualized in Thymocytes and T Cells.

Authors - Bonnie Yen
View Article