IL-10RB Polyclonal antibody

IL-10RB Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15102-1-AP

Synonyms

CDW210B, CRF2 4, CRFB4, D21S58, D21S66, IL 10 receptor subunit beta, IL 10R subunit 2, IL 10R subunit beta, IL 10R2, IL 10RB, IL10RB, IL-10RB, IL-10Rβ, interleukin 10 receptor, beta



Tested Applications

Positive WB detected inrat skeletal muscle tissue, Jurkat cells, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15102-1-AP targets IL-10RB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-10RB fusion protein Ag7445
Full Name interleukin 10 receptor, beta
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC001903
Gene Symbol IL10RB
Gene ID (NCBI) 3588
RRIDAB_10638926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RB, also known as IL-10R2 or CDw210b, is the signaling subunit of the IL-10R complex and is constitutively expressed in a variety of cell types (PMID: 24507158). Binding of IL-10 to the IL-10R complex leads to JAK1- and TYK2-mediated phosphorylation of STAT3 (PMID: 11244051; 10433356). IL-10RB is also shared by several other cytokines, including IL22, IL-26, IFN-γ, IL-28A/B and IL29 (PMID: 22236434).

Protocols

Product Specific Protocols
WB protocol for IL-10RB antibody 15102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Mol Basis Dis

IL-22 ameliorated cardiomyocyte apoptosis in cardiac ischemia/reperfusion injury by blocking mitochondrial membrane potential decrease, inhibiting ROS and cytochrome C.

Authors - Yang Che
mouseWB

J Alzheimers Dis

Regulation of the Human IL-10RB Gene Expression by Sp8 and Sp9

Authors - Shuyue Luo
mouseWB

PLoS One

Endogenous interleukin-22 prevents cardiac rupture after myocardial infarction in mice

Authors - Mai Yamamoto